منابع مشابه
TUNEL vision spots apoptotic cells
By the early 1990s, scientists knew that programmed cell death, or apoptosis, was important for tissue development and homeostasis as well as for cancer progression. Because apoptotic cells activate nucleases to cleave their chromatin, cell death could be followed in vitro by extracting DNA from a population of tissue culture cells and running it out on an agarose gel to look for a characterist...
متن کاملApoptotic cells in the human endometrium and placental villi: pitfalls in applying the TUNEL method.
Apoptotic cells were histochemically demonstrated by the TdT-mediated biotinylated dUTP nick end-labeling (TUNEL) method in formalin-fixed and paraffin-embedded sections of the human endometrium and placental villi. In 53 endometrial biopsy specimens, labeled nuclei were identified in 16 samples showing a desquamating change, associated with menstruation, functional bleeding or adenocarcinoma. ...
متن کاملAmplification of mRNAs from single, fixed, TUNEL-positive cells.
her, J.J. McCormick, G.P. Taylor and R.J. Klebe. 1998. Overview of matrix metalloproteinase expression in transformed cells. Matrix Biol. 16:483-496. 3.Hill, H.Z., C. Huselton, B. Pilas and G.J. Hill. 1987. Ability of melanins to protect against the radiolysis of thymine and thymidine. Pigment Cell Res. 1:81-86. 4.Kreader, C.A. 1996. Relief of amplification inhibition in PCR with bovine serum a...
متن کاملIncreased TUNEL positive cells in human alcoholic brains.
Alcohol-sensitive neuronal cell loss, which has been reported in the superior frontal cortex and hippocampus, may underlie the pathogenesis of subsequent cognitive deficits. In the present study, we have used the TUNEL labeling to detect the DNA damage in human alcoholic brains. Seven out of eleven alcoholics exhibited TUNEL-positive cells in both superior frontal cortex and hippocampus, which ...
متن کاملStudy of the Effect of Hypothyroidism on the Apoptotic Index in Rat Ovarian Follicles, Using the TUNEL Technique
Introduction: Among the endocrine diseases, hypothyroidism is the commonest after diabetes.Thyroid hormones (T3,T4) are essential for genital organs function. Apoptosis process in ovarian cells plays a significant role of development in ovarian follicles. The aim of the present study was determine the apoptotic index induced by hypothyroidism in rat ovarian follicles. Materials and Method...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 2015
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.2081fta